Chemical and enzymatic changes associated with mouse liver necrosis in vitro.

Necrosis and cell death occur frequently in vari ous pathological conditions, including neoplasia; many tumors are composed of mixtures of neoplastic and non-neoplastic cells, necrotic cells, stremai, inflammatory, and blood cells. These cells, depending on metabolic factors and nutri tion, are in quite different phases of activity rang ing from the rapid growth of the neoplastic and regenerative states to the degeneration character istic of the necrotic state. In such mixtures of cells it is not feasible by present technics to characterize the metabolic activity of each type of cell. The development of neoplasia and death may be regarded as progressive states which at some stages may be reversible; the exact stage of de marcation in the progression of a normal cell to the "irreversible" state of neoplasia or death cannot